Human zona proteins are incorporated into the zona pellucida of transgenic mouse eggs. Ovulated eggs from human ZP1 (huZP1), huZP2, and huZP3 rescue as well as huZP4 transgenic mice were fixed and stained with mAb to human ZP1 (huZP1), huZP2, huZP3, huZP4, mouse ZP1 (moZP1), moZP2, and moZP3. Antibody binding to human and mouse zona proteins was detected by confocal microscopy and faux colored dark blue (huZP1), red (huZP2), green (huZP3), orange (huZP4), magenta (moZP1), light blue (moZP2), or yellow (moZP3). Fluorescent and DIC images were merged.