Arp2/3 complex inhibition does not affect myosin II localization. (A) Fluorescent labeling of growth cones with myosin II antibody (right) and TRITC-phalloidin (left) after live cell extraction. Growth cones were treated with vehicle (DMSO, 20 min), CK666 (100 µM, 20 min), blebbistatin (60 µM, 20 min), or pretreated with blebbistatin (60 µM, 10 min) followed by blebbistatin and CK666 (100 µM, 20 min). (B) Line scan analysis of myosin II localization in growth cones under each condition in A. Line scans (50 pixels in width, 2x P-domain in length) were sampled along the growth axis of growth cones. Scattered dots represent dataset from individual growth cones. Solid lines represent the population average. n, growth cones measured. Yellow dotted lines demarcate leading edge. Bars, 10 µm.