Figure 4.

DRP1 down-regulation inhibits cell cycle exit of mitotic follicle cells. (A) Mitochondria (HSP-60 labeled) are more clustered in clones of drp1KG (lacking UbiGFP) than in nonclonal cells in an S5 mitotic egg chamber. Bottom row shows magnification. Nuclei are stained with Hoechst. (B) Increased pH3 labeling occurs in drp1KG clones (lacking UbiGFP) in the S5 egg chamber. (C) Quantification of pH3-positive cells in drp1KG clones versus wild-type (WT) background in all mitotic stages. (D) Marf-1 RNAi clones (CD8GFP positive) have increased Hnt and HSP-60 immunolabeling relative to surrounding cells in an S5 mitotic egg chamber. (E) drp1KG clones expressing Marf-1 RNAi (CD8GFP positive) show no increase in Hnt or HSP-60 immunolabeling in an S3 mitotic egg chamber. The white lines define the clone boundary, and the dotted lines outline the egg chambers. Bars, 10 µm.

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