Figure 3.
Figure 3. ARPC3−/− fibroblast cells in wound healing and their leading edge dynamics. (A and B) Wound-healing montage of ARPC3+/+ and ARPC3−/− fibroblast cells. Bars, 50 µm. (C) Quantification of wound area as a function of time. Plots show mean and standard deviation from six different regions along the wound per experiment and three experiments per genotype. (D) Cell boundary segmentation and illustration of leading edge kymograph line selection. Bars, 25 µm. (E) Each kymograph (left) was digitized (center) and then smoothed for assignment of protrusion and retraction segments. (F) Angular correlation functions were computed as described in Materials and methods. (G) The smaller width at half maximum of the angular correlation function for mutant indicates less coordinated motion of the leading edge than for wild type. Small box shows the mean, line shows median, large box the SEM, and whiskers show SD.

ARPC3−/− fibroblast cells in wound healing and their leading edge dynamics. (A and B) Wound-healing montage of ARPC3+/+ and ARPC3−/− fibroblast cells. Bars, 50 µm. (C) Quantification of wound area as a function of time. Plots show mean and standard deviation from six different regions along the wound per experiment and three experiments per genotype. (D) Cell boundary segmentation and illustration of leading edge kymograph line selection. Bars, 25 µm. (E) Each kymograph (left) was digitized (center) and then smoothed for assignment of protrusion and retraction segments. (F) Angular correlation functions were computed as described in Materials and methods. (G) The smaller width at half maximum of the angular correlation function for mutant indicates less coordinated motion of the leading edge than for wild type. Small box shows the mean, line shows median, large box the SEM, and whiskers show SD.

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