Proteomic identification of integrin receptors as cargoes for SNX17. (A) The table lists transmembrane proteins that were at least threefold less abundant in SNX17-depleted HeLa cells compared with control. The light/medium is the ratio between the abundance of the respective protein in control cells compared with SNX17-depleted cells. Light/heavy is the ratio between the detected proteins in control versus SNX27-suppressed cells. (B) Overnight treatment with 100 nM bafilomycin A prevents loss of integrins in SNX17- and SNX27-depleted cells. (C) Western blot showing the effect of four different SNX17 siRNAs and a pool (SMARTpool [sp]) of these four oligos on Itgα5/β1 levels and the effect of bafilomycin treatment. (D) Quantitative Western blot analysis of the effect of SNX17 suppression on total Itgα5 and β1 levels. The graph represents the mean of five independent experiments. (E) Quantitative analysis of the effect of SNX17 suppression on the mature (130 kD) and the immature (100 kD) forms of Itgβ1. The graph represents the mean of three independent experiments. (F and G) Bafilomycin rescue of the loss of Itgα5β1 in SNX17-depleted cells. The graphs show the mean of four independent experiments. *, P < 0.05. IB, immunoblot. Error bars indicate the standard deviation.