Figure 5.
Figure 5. Sey1p dimerizes in the presence of GDP AlFx. Analytical ultracentrifugation was used to calculate the c(s) distributions obtained for Sey1-ΔTM in the absence of nucleotide (blue) and in the presence of either 2 mM GDP (red) or 2 mM GDP, 2 mM AlCl3, and 20 mM NaF (green). In the absence of nucleotide or presence of GDP, a molecular mass of 90 kD was determined for the major species at 3.85 S, consistent with a Sey1-ΔTM monomer (calculated molecular mass = 85.697 kD). In the presence of GDP AlFx, the major species at 6.31 S had a molecular mass of 186 kD.

Sey1p dimerizes in the presence of GDP AlFx. Analytical ultracentrifugation was used to calculate the c(s) distributions obtained for Sey1-ΔTM in the absence of nucleotide (blue) and in the presence of either 2 mM GDP (red) or 2 mM GDP, 2 mM AlCl3, and 20 mM NaF (green). In the absence of nucleotide or presence of GDP, a molecular mass of 90 kD was determined for the major species at 3.85 S, consistent with a Sey1-ΔTM monomer (calculated molecular mass = 85.697 kD). In the presence of GDP AlFx, the major species at 6.31 S had a molecular mass of 186 kD.

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