The impact of DDB2 deficiency and ATP depletion on XPC recruitment to DNA damage. (A and B) Wild-type VH10 cells (A) or XP-E (GMO1389-TERT) cells (B) were either mock treated (top rows) or ATP depleted (bottom rows), locally UV irradiated (25 J/m² through 8-µm pores), and subsequently stained for endogenous XPC (green) and CPDs (red). (C and D) Wild-type VH10 cells (C) or XP-E (GMO1389-TERT) cells (D) were either mock treated (top rows) or ATP depleted (bottom rows), locally UV irradiated (50 J/m² through 8-µm pores), and subsequently stained for endogenous XPC (green) and CPDs (red). (E and F) The quantification of the relative accumulation of XPC (red bars) or the enrichment of CPDs (blue bars) is shown in E (25 J/m²) and F (50 J/m²). The signal represents the relative increase at the locally damaged site relative to the signal in the nondamaged nuclear region (∼50 cells for each condition collected in two independent experiments). Error bars represent the SD. depl, depleted.