UV-induced chromatin changes require DDB2. (A–C) Distribution of GFP-H4 at sites of local damage (100 J/m2 through 5-µm pores) in a DDB2-deficient XP-E cell line XP23PV (A), an XP-E cell line complemented by transient expression of DDB2-mCherry to restore the DDB2 deficiency (B), and an XP-C cell line (C). (D) Quantification of the intensity of GFP-tagged histones at sites of UV-induced DNA damage shown in a box plot (∼50 cells for each condition). The dotted line represents a ratio of 1, which means that the intensity in the local damage is the same as the intensity elsewhere in the nucleus. Asterisks indicate significant differences based on a t test (P < 0.05). Open circles are data points that are considered outliers, as their value is greater than the upper quartile, +1.5 the interquartile distance, or less than the lower quartile, −1.5 the interquartile distance. (E) Distribution of GFP-H4 at the LacO array in AO3 cells upon tethering LacR-DDB2.
