Minimal DDB2 domain that is sufficient for chromatin unfolding. (A) A schematic representation of DDB2 deletion mutants analyzed. (B) Expression of mCherry-LacR–tagged DDB2 proteins in the absence (−) or presence (+) of proteasome inhibitor MG132 (10 µM). WB, Western blot; WT, wild type. (C–H) Images show a confocal slice (1 Airy unit) of the distribution of the indicated mCherry-LacR fusion proteins in AO3 cells. The lookup table is shown. (I) Quantification of array decondensation in AO3 cells (in ratio array/nucleus) after tethering the indicated LacR-DDB2 fusion proteins shown in a box plot. Values represent the mean array decondensation of 50–100 cells collected in two independent experiments. Open circles are data points that are considered outliers, as their value is greater than the upper quartile, +1.5 the interquartile distance, or less than the lower quartile, −1.5 the interquartile distance.