Figure 1.
Figure 1. Functional tethering of DDB2. (A) A schematic representation of murine DDB2 including mutations found in XP-E patients (in red). The seven WD40 β-propellers (1–7) and α-helical domain (α) are indicated. (B–E) Recruitment of GFP-DDB1 and GFP-CUL4A to tethered mCherry-LacR-DDB2 (B), mCherry-LacR-DDB2K244E (C), mCherry-LacR-DDB2D307Y (D), or mCherry-LacR-DDB2L350P (E). (F) Expression of GFP-DDB2 proteins in the absence (−) or presence (+) of proteasome inhibitor MG132 (10 µM). WB, Western blot; WT, wild type. (G–J) FRAP analysis of GFP-DDB2 (G), GFP-DDB2K244E (H), GFP-DDB2D307Y (I), or GFP-DDB2L350P (J) in unchallenged cells (blue lines) or globally (16 J/m2) UV-irradiated cells.

Functional tethering of DDB2. (A) A schematic representation of murine DDB2 including mutations found in XP-E patients (in red). The seven WD40 β-propellers (1–7) and α-helical domain (α) are indicated. (B–E) Recruitment of GFP-DDB1 and GFP-CUL4A to tethered mCherry-LacR-DDB2 (B), mCherry-LacR-DDB2K244E (C), mCherry-LacR-DDB2D307Y (D), or mCherry-LacR-DDB2L350P (E). (F) Expression of GFP-DDB2 proteins in the absence (−) or presence (+) of proteasome inhibitor MG132 (10 µM). WB, Western blot; WT, wild type. (G–J) FRAP analysis of GFP-DDB2 (G), GFP-DDB2K244E (H), GFP-DDB2D307Y (I), or GFP-DDB2L350P (J) in unchallenged cells (blue lines) or globally (16 J/m2) UV-irradiated cells.

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