Levels of γ-tubulin, nuclear receptors, and SPB core proteins during MT nucleation. (A) MT renucleation assay of TUB4-yeGFP mCherry-TUB1 cells. Nocodazole-arrested cells were released from the cell cycle block by washing the cells to remove nocodazole. The removal of nocodazole triggered MT renucleation. The relative fluorescence intensities (rel. fl. int.) of Tub4-yeGFP were quantified during the time of MT regrowth. The graph on the left shows relative intensities of Tub4-yeGFP at SPBs. Note that the Tub4-yeGFP SPB signal intensity is not changing during the experiment. Deconvolved images of the same cell are shown on the right. One representative experiment is shown. mSPB, mother SPB; dSPB, daughter SPB. (B) MT renucleation assay of SPC110-yeGFP, Spc42-yeGFP, and TUB4-yeGFP cells. Experiments were performed as in A. One representative experiment for each cell type is shown. Note that cells in B do not contain mCherry-TUB1. (A and B) Bars, 5 µm.