Levels of γ-TuSC and receptors during the cell cycle. (A, left) The signal intensity of the indicated yeGFP-tagged γ-TuSC proteins was analyzed by time-lapse analysis (graph of mean relative intensity with SD error bars) n is the number of cells, and t = 0 min indicates the time of SPB separation. The time of SPB duplication is indicated from t = −20 to t = 0 min. During this time interval, the measured signal is derived from two SPBs. dSPB, daughter SPB; mSPB, mother SPB. (right) Images from time-lapse videos of representative cells expressing the gene fusion indicated in the figure. (B) As in A but with SPC72-yeGFP or SPC110-yeGFP. (A and B) rel. fl. int., relative fluorescence intensity. Bars, 2 µm.