Figure 6. Estimated stoichiometry of... | Rockefeller University Press

Figure 6.

$Figure 6. Estimated stoichiometry of clathrin coats. To estimate the abundance of major CCV proteins in the CCV fraction, emPAIs were calculated from the SILAC data (Table S1) and normalized to CHC (relative emPAI = 1). The approximate number of copies of each protein in a “typical” CCV with 40 triskelia (Cheng et al., 2007) is also indicated. Error bars indicate SD (n = 3). (A) Relative abundance of APs and clathrin. For every CHC molecule, there are ∼0.3 light chains (CLT A + B) and ∼0.3 AP complexes. AP-1 is approximately twice as abundant as AP-2, which suggests that ∼2/3 of the CCVs in the preparation are intracellular, and ∼1/3 are endocytic. (B) Estimated composition of the intracellular CCV coat, assuming a CHC/AP-1 ratio of ∼3:1. There may be more than one type of intracellular CCV; the figure shows the estimates for a hypothetical average CCV. (C) Estimated composition of the endocytic CCV coat, assuming a CHC/AP-2 ratio of ∼3:1. Asterisks indicate shared proteins.$

Estimated stoichiometry of clathrin coats. To estimate the abundance of major CCV proteins in the CCV fraction, emPAIs were calculated from the SILAC data (Table S1) and normalized to CHC (relative emPAI = 1). The approximate number of copies of each protein in a “typical” CCV with 40 triskelia (Cheng et al., 2007) is also indicated. Error bars indicate SD (n = 3). (A) Relative abundance of APs and clathrin. For every CHC molecule, there are ∼0.3 light chains (CLT A + B) and ∼0.3 AP complexes. AP-1 is approximately twice as abundant as AP-2, which suggests that ∼2/3 of the CCVs in the preparation are intracellular, and ∼1/3 are endocytic. (B) Estimated composition of the intracellular CCV coat, assuming a CHC/AP-1 ratio of ∼3:1. There may be more than one type of intracellular CCV; the figure shows the estimates for a hypothetical average CCV. (C) Estimated composition of the endocytic CCV coat, assuming a CHC/AP-2 ratio of ∼3:1. Asterisks indicate shared proteins.