Figure 2. CCV proteins and contaminants... | Rockefeller University Press

Figure 2.

$Figure 2. CCV proteins and contaminants have distinct profiles. The fold changes of selected CCV proteins (AP-1 and AP-2 subunits) and copurifying proteasomal contaminants (PSMA1 and PSMB1) are shown for 10 SILAC datasets. Proteins that are subunits of the same protein complex have closely correlated profiles. Con/Cla kd 1–3: Triplicate repeats of control versus clathrin-depleted. Aux kd/Con 1–3: Triplicate repeats of auxilin-depleted versus control. Fractionation 1–3: Triplicate repeats of improved versus original CCV preparation. Fractionation 4*: A further comparison based on fractionation properties of CCVs (see Fig. S1, D and E).$

CCV proteins and contaminants have distinct profiles. The fold changes of selected CCV proteins (AP-1 and AP-2 subunits) and copurifying proteasomal contaminants (PSMA1 and PSMB1) are shown for 10 SILAC datasets. Proteins that are subunits of the same protein complex have closely correlated profiles. Con/Cla kd 1–3: Triplicate repeats of control versus clathrin-depleted. Aux kd/Con 1–3: Triplicate repeats of auxilin-depleted versus control. Fractionation 1–3: Triplicate repeats of improved versus original CCV preparation. Fractionation 4*: A further comparison based on fractionation properties of CCVs (see Fig. S1, D and E).