L167E + L170E (LL) mutation in Num1 specifically disrupts dynein-anchoring but not patch assembly function. (A) The percentage of cells exhibiting stationary cortical Dyn1-3GFP foci. Error bars are standard error of proportion (n = 179 for NUM1, 500 for num1^LL, and 539 for num1^EK). (B) Single–focal plane image of live cells expressing chromosomally tagged Num1^LL-GFP. (C) num1^LL cells expressing Dyn1-3GFP and mCherry-Tub1 or Jnm1-3mCherry and CFP-Tub1 showing loss of cortical dynein and dynactin foci. Each image is a maximum intensity projection of a 2-µm z stack of widefield images. Dotted lines outline the cell. Bars, 1 µm.