Aurora B phosphorylation regulates the function and localization of the Ska complex. (A) A schematic representation of the Ska rescue protocol to assess mitotic progression. A box-and-whisker plot showing the elapsed time (minutes) that cells spent in mitosis from NEBD to anaphase onset or mitotic cell death (≥60 cells from two independent experiments) is shown. Percentages of mitotic cell death and cells with misaligned chromosomes are displayed below the plot. (B) HeLa S3 cells were treated as in A but fixed after a 10-h release from the second thymidine block. (C) Cells were treated as in B, but Mis12-INCENP-GFP WT or TAA plasmids were cotransfected with the Ska plasmids. Bars, 10 µm.