Aurora B antagonizes Ska complex localization to KTs. (A) HeLa S3 cells were either treated with 3.3 µM nocodazole (Noc) for 1 h before a 2-h incubation at 4°C (Cold) with ZM447439 (ZM) or left untreated. (B) A bar graph showing the quantification of Ska3 staining intensity at KTs (normalized against CREST) of cells treated as in A (>100 KTs from five cells; error bars indicate the SD of five cells). a.u., arbitrary unit. (C) GFP^LAP-Ska2–expressing HeLa cells were treated with 3.3 µM nocodazole for 1 h followed by 2 h with DMSO, ZM, or cold. (D) HeLa S3 cells were transfected with plasmids encoding GFP alone, Mis12-INCENP-GFP WT, or TAA. (E) A bar graph showing the quantification of Ska3 staining intensity at KTs (normalized against CREST) of cells treated as in D (>100 KTs from five cells; SD of five cells). Bars, 10 µm.