Figure 3. Thrombin induces FAJs... | Rockefeller University Press

Figure 3.

$Figure 3. Thrombin induces FAJs formation by transformation of stable AJs. (a) Time-lapse images of HUVECs expressing p120-catenin–mCherry (red) and Vinculin-GFP (green) after thrombin stimulation. Merged images on the right highlight the rapid recruitment of Vinculin during thrombin-induced junction remodeling at the region of interest. See corresponding Video 4 for the ∼1-h time-lapse recording. (b) Time-lapse images of HUVECs expressing α-catenin–Dendra2 before and after photoswitching a fraction of a stable junction using a 405-nm confocal laser followed by thrombin-induced FAJ formation. See corresponding Video 5 for an 8-min time-lapse recording. Bars: (a, left) 10 µm; (a, right) 5 µm; (b) 10 µm$

Thrombin induces FAJs formation by transformation of stable AJs. (a) Time-lapse images of HUVECs expressing p120-catenin–mCherry (red) and Vinculin-GFP (green) after thrombin stimulation. Merged images on the right highlight the rapid recruitment of Vinculin during thrombin-induced junction remodeling at the region of interest. See corresponding Video 4 for the ∼1-h time-lapse recording. (b) Time-lapse images of HUVECs expressing α-catenin–Dendra2 before and after photoswitching a fraction of a stable junction using a 405-nm confocal laser followed by thrombin-induced FAJ formation. See corresponding Video 5 for an 8-min time-lapse recording. Bars: (a, left) 10 µm; (a, right) 5 µm; (b) 10 µm