Model of Gish/Rab11 vesicle trafficking during trichome formation. (A) Model of gish-mediated vesicle trafficking during trichome formation. During prehair formation, two systems exist along orthogonal axes: (1) proximally enriched Mwh restricts actin nucleation along the proximal–distal axis, and (2) Gish directs the polarized trafficking and enrichment of Rab11 endosomes (and derived vesicles) to the prehair. Targeting of vesicle recycling restricts actin nucleation to a single site within the distal cell vertex. Polarized vesicle recycling to the proximity of the prehair base provides a mechanism whereby nucleation (yellow) is prevented from diffusing laterally, resulting in multiple trichomes. (B) Loss of Rab11–Nuf–Sec15 function results in reduced, absent, or abnormal trichome formation. (C) In gish mutant cells, focused Rab11–Nuf–Sec15 recycling is disrupted, resulting in diffuse membrane trafficking of nucleation activity and multiple trichome formation. EE, early endosome; RE, recycling endosomes.