CK1-γ/gish regulates Rab11 effector Sec15GFP. (A–A′′) Endogenous Sec15 (green and monochrome) is reduced in apical gish^IR tissue (blue; yellow lines mark clone border). (B–C′) nub-gal4 UAS-gish^IR disrupts Sec15GFP aggregates in 30-h APF pupal wings (C and C′) compared with nub-gal4 control tissue (B and B′; Fmi in magenta marks the membrane). (D and D′) The mwh¹-null allele has no effect on Sec15GFP aggregates. (E) Quantification of the mean number of Sec15GFP puncta per cell. Error bars indicate SDs; unpaired t testes were performed on three independent animals (**, P < 0.01). (F and F′) nub-Gal4 UAS-Sec15GFP expression in 30-h APF wings reveals large punctate colocalization of Sec15GFP and Rab11 (blue and monochrome in F and F′). (G and G′) nub-Gal4 coexpression of UAS-Sec15GFP and UAS-gish^IR reveals dissociation of both Sec15GFP and Rab11 (blue and monochrome in G and G′). Bars, 10 µm.