Rab11 and effectors are required for trichome formation. (A–A′′) 32–34-h APF pupal wings of actin-Gal4 FLP-out clones expressing UAS-YFP-Rab11^DN (green; yellow lines mark clone border in A′ and A′′) display cells with short/missing prehairs (yellow asterisks in A′ and A′′; prehairs are red in A and A′ and monochrome in A′′). Cells are outlined with Fmi (blue in A and A′). (B and B′) 30–32-h APF wings of actin-Gal4 FLP-out clones expressing UAS-gish^IR (blue; yellow line marks clone border) reveal dissociated Nuf foci (green and monochrome in B and B′) associated with the base of developing trichomes (red; yellow arrowheads indicate Nuf enriched at the base of the prehair). (C) en-Gal4 UAS-dicer2 UAS-nuf^IR adult wings display short (red arrowheads) or malformed (red asterisks) trichomes. (D) Quantification of the Rab11 and nuf LOF phenotypes was performed on three independent animals; error bars indicate SDs. (E) Control tissue adjacent to the dpp domain of Sec15^IR expression exhibits focused Rab11 localization (green) at the trichome base (red; yellow arrowheads indicate Rab11 enriched at the base of the prehair). (F–F′′) 32–34-h APF pupal wing expressing dpp>Sec15^IR after Gal80^ts temperature shift in third instar displays delayed or missing trichomes (red and monochrome in F and F′; yellow asterisks highlight examples of missing or shortened prehairs; the yellow arrowhead indicates an occasional multiple prehair) and accumulated/mislocalized endogenous Rab11 (green and monochrome in F and F′′). Bars: (A, B, E, and F) 10 µm; (C) 25 µm.