CK1-γ/Gish is associated with the base of the developing trichome. (A and A′) 32–34-h APF wing actin-Gal4 FLP-out clones expressing UAS-gish^IR (blue; yellow line marks clone border). Endogenous Gish (green and monochrome in A and A′) is associated with the prehair base (prehair labeled with rhodamine-phalloidin, red). (B and B′) Magnified view of A and A′, outside UAS-gish^IR expression clone. (C–D′) 32–34-h APF pupal wings expressing UAS-myc-gish^WT or UAS-myc-gish^ΔC in actin-Gal4 FLP-out clones labeled with anti-Myc (green) and rhodamine-phalloidin (red). (C and C′) Gish is observed upon expression of Myc-Gish^WT (green or monochrome in C or C′). (D and D′) C-terminal truncation, Myc-Gish^ΔC, is ubiquitously localized (green or monochrome in D or D′). (E and F) Basal projections of punctate and membrane localization of endogenous Gish (E) or Myc-Gish^WT (F) proteins. Bars: (A) 10 µm; (B–F) 5 µm.