Figure 2.

Trajectory of a sperm cell in a radial gradient of resact is determined by the time derivative of [Ca2+]i. (A) Swimming path of a sperm cell in a radial gradient of resact. The UV profile used for uncaging resact is indicated by shades of gray. The cell swims on circles drifting up the gradient while [Ca2+]i oscillates. Arrows indicate the swimming direction. (B) Changes in Fr and path curvature κ oscillate with identical frequency, but the oscillations do not superimpose. (C) Changes in dFr/dt and path curvature κ superimpose and are strongly correlated. (D) Pearson correlation between path curvature and dFr/dt or Fr. Individual data points are displayed as black circles (n = 27), mean correlation is in red, and SD is in blue (box length is twice the SD).

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