Importin-β 45–462 induces mitotic dynamic defects in time-lapse recorded HeLa cells. Still images from video-recorded cells transfected with importin-β 45–462 and H2B-GFP, displaying unstable chromosome alignment (cell a) and spindle axis rotation (cell b, the double-arrowed red line represents the major spindle axis). Images were taken under an inverted microscope (TE300, Nikon; wide-field channel every 2 min, fluorescence channel every 30 min). Bars, 10 µm.