Phenotypic heat maps of the Nup84 complex: Fitness relates with loss of interactions between the Nup84 complex and other core NPC nups, whereas NPC clustering is related to NE membrane stability. (A and B) Effect of truncated regions of the Nup84 complex on organism fitness and NPC clustering. The severity of fitness phenotypes (A) and NPC clustering (B) for specific truncations of the Nup84 complex are shown mapped into a single Nup84 complex structure. The color code is shown in between the maps and in Fig. S3. (C) Membrane-destabilizing assay. 0.4% BA was added to wild-type (wt) or truncated mutant Nup133(1–898) (similar behavior was observed for other mutant strains; not depicted). The time course localization of fluorescent reporter Nup49-CFP after treatment and for nontreated controls is shown on the top. Quantification of the level of NPC clustering (see Materials and methods) for each time point is shown on the bottom (mean ± SD; n = 30). Bar, 5 µm.