Density map for the Nup84 complex ensemble. (A) Two views of the localization density map are shown for the ensemble. The gray outer envelope represents the density within which 90% of all Nup84 complex structures in the final ensemble were localized, and the colored inner envelope was thresholded by visual inspection (to a volume containing 37% of ensemble density) to match the volume of an individual structure (also see Video 1). A few example structures that best fit the inner envelope according to 3D cross-correlation score and have among the lowest C-α RMSDs from the average structure comprised of the 9,479 final good-scoring solutions are shown. (B) Two views of the density map, containing two fitted ribbon structures from the ensemble.