Identification of interacting regions within the Nup84 complex. (A) Copurification profile of the different truncations analyzed. Horizontal gray lines represent the amino acid residue length of each protein and truncated version; amino acid residue positions are shown on top of the lines. On the right, MS-detected copurifying Nup84 complex nups are indicated (+), and undetected proteins are also indicated (−; see Table S3 for details). (B) Domain mapping. Truncations showing both nuclear rim localization by immunofluorescence and PAL profile consistent with proper folding were used to interpret a lost interaction as caused by the loss of at least one interaction point present in the deleted nup region (represented by red lines on the wild-type line). Each region is identified by red roman numbers. The identified interactions are as follows: Nup133 Region I interacts with Nup120, Nup145c, Nup85, Nup84, Seh1, and Sec13; Nup120 Region II interacts with Nup133, Nup145c, Nup85, Nup84, Seh1, and Sec13; Nup85 Region III interacts with Seh1; Nup85 Region IV interacts with Nup133, Nup120, Nup145c, Nup84, and Sec13; Nup84 Region V interacts with Nup133, Nup120, Nup145c, Nup85, Seh1, and Sec13; Nup145c Region VI interacts with Nup120, Nup85, and Seh1; Nup145c Region VII interacts with Sec13; Nup145c Region VIII interacts with Nup120, Nup85, and Seh1; Nup145c Region IX interacts with Nup133 and Nup84; Nup145c Region X interacts with Sec13; and Nup145c Region XI interacts with Nup120, Nup85, and Seh1.