Domain mapping of the Nup84 complex. (top) Predicted secondary structure of truncated nup constructs. Vertical lines represent the sequence of each nup to scale, and secondary structure predictions are shown as horizontal bars of length proportional to the confidence of the prediction (α helices are shown in magenta and β-strands in cyan). PAL sites (Dokudovskaya et al., 2006) defining the limits of the truncations are indicated with arrowed black letters; truncation points selected by sequence alignment are indicated with arrowed green letters. (bottom) Coomassie-stained SDS-PAGE of affinity-purified PrA-tagged truncated Nup84 complex nups. Protein bands marked by filled circles at the left side of the gel lanes were identified by MS. The identity of the copurifying proteins is indicated in order below each lane (PrA-tagged nups are shown in blue, copurifying nups in black [N = Nup, S1 = Seh1, and S13 = Sec13], and contaminants in orange). WT, wild type.