Autophagosomes along the axon have fused with late endosomes or lysosomes. (A) GFP-LC3 and LAMP1-RFP distribution at the distal end of the neurite. Yellow, green, and red arrowheads designate vesicles positive for both markers, GFP-LC3 only, or LAMP1-RFP only, respectively. (B) Time series and corresponding kymograph of GFP-LC3 and LAMP1-RFP motility along the axon. Open, cyan, and dark blue arrowheads denote three different examples of vesicles positive for both markers. Yellow arrowheads in kymograph denote multiple examples of vesicles positive for both markers. (C) GFP-LC3 and LysoTracker (LysoT) red localization at the neurite tip. Yellow, green, and red arrowheads designate vesicles positive for both markers, GFP-LC3 only, or LysoTracker red only, respectively. (D) Time series and corresponding kymograph of GFP-LC3 and LysoTracker red motility along the axon. Open arrowheads denote a vesicle positive for both markers. Yellow arrowheads in kymograph denote multiple examples of vesicles positive for both markers. Horizontal bars: (distal images) 1 µm; (axon images) 10 µm. Vertical bars, 1 min.