The maturation of focal adhesions into tensin-rich fibrillar adhesions is abrogated in the absence of RSFs. (A) Representative immunofluorescence TIRF images of Pxn and tensin along with the corresponding confocal actin image for WT, Dia Inh, and Atn-1 KD fibroblasts on fibronectin-coated glass coverslips. Bar, 10 µm. (B) Representative immunofluorescence actin, Pxn, and fibronectin images for WT, Dia-inhibited, and Atn-1 KD fibroblasts on fibronectin-coated glass coverslips 24 h after plating. The arrows indicate representative areas of fibronectin remodeling. Bar, 50 µm.