Nuclear export prevents rereplication. (A) Time-lapse images of embryos at 3, 4, and 5 min after anaphase onset of meiosis II. Embryos are expressing GFP–ORC-2 (left images in each panel) and mCherry-Histone (right images in each panel). Wild type (left), npp-8 RNAi (middle), and xpo-1 RNAi (right) are shown. Bar, 5 µm. See also Video 7. (B) Chromatin enrichment of GFP–ORC-2 in wild-type and xpo-1 RNAi embryos. (C) Mean time for GFP–ORC-2 chromatin enrichment to persist after anaphase of meiosis and mitosis (mean of five embryos). (D) Accumulation of mCherry-Histone during metaphase of the first mitosis in wild-type (top) and xpo-1 RNAi (bottom) embryos. The mean mCherry fluorescence intensity of five embryos is shown. (A and D) Asterisks indicate polar bodies. Arrows point to the position of the chromatin used for quantification. (E) Visualization of chromatin in ∼20 cell stage embryos fixed and stained with antitubulin and a DNA dye. (C and D) Error bars represent SD. (D and E) Bars, 10 µm.