GFP–CDC-6 and CDT-1 localization. (A–C) Images from time-lapse sequences of embryos expressing GFP–CDC-6 (left images in each panel) and mCherry-Histone (right images in each panel), presented as for Fig. 1. Asterisks indicate polar bodies. (A) Wild-type embryo. Red arrowheads indicate the enrichment of GFP–CDC-6 on chromatin in anaphase of meiosis I and prometaphase of first mitosis. Green arrowheads show the position of interphase nuclei. (B) orc-5 RNAi embryo. (C) cdt-1 RNAi embryo. (D) Mean chromatin enrichment in five embryos according to cell cycle stage. Error bars are SDs. See also Video 6. (E and F) CDT-1 localization during metaphase and anaphase of meiosis II. Wild-type (E) and mcm-7 RNAi (F) embryos stained with anti–CDT-1 and antitubulin antibodies are shown. The arrow indicates chromatin-bound CDT-1. Insets to the left show a 2.5-fold magnification of maternal chromosomes. Bars, 10 µm, except for the insets in E and F, in which the bars are 5 µm.