GFP–ORC-2 and replication origins. (A–D) Images from time-lapse sequences of embryos expressing GFP–ORC-2 (left images in each panel) and mCherry-Histone (right images in each panel), presented as for Fig. 1. (A) Wild-type embryo. Red arrowheads indicate the enrichment of GFP–ORC-2 on chromatin in anaphase of meiosis I and prometaphase of first mitosis. Green arrowheads show the position of interphase nuclei. (B) orc-5 RNAi embryo. (C) orc-1 RNAi embryo. (D) cdt-1 RNAi embryo. (E) GFP–ORC-2 chromatin enrichment (the mean of five embryos at various cell cycle stages). Error bars are SDs. (F) Embryos expressing GFP–ORC-1 and mCherry-Histone. Asterisks (A–C, F) indicate polar bodies. Bars, 10 µm. See also Videos 4 and 5.