dEHBP1 is transiently enriched at the interface of the pIIa/pIIb cells, where it partially colocalizes with Spdo. (a–d) Still frames from Video 1 showing medial sections of pIIa/pIIb cells that contain mCherry-dEHBP1 in intracellular punctae, pointed by arrows, as well as the interface of the cells, indicated by arrowheads. Numbers at the top right corner indicate the minutes of live imaging. (e–k) Still frames from Videos 2 and 3 showing apical and medial sections of pIIa/pIIb cells, respectively, that express mCherry-dEHBP1 and Spdo-GFP, which colocalize along the interface of dividing pII cells toward the subapical regions (i–k). Numbers at the top right corner indicate the minutes of live imaging. (i–k) Magnification of the pIIa/pIIb cells included in the box in g. (l–r) mCherry-dEHBP1 (arrow) and Spdo GFP punctae (arrowheads) do not colocalize, but only at the interface of dividing pII cells (p–r). (p–r) Magnification of the pIIa/pIIb interface included in the box in m. Single-channel representations are shown in black and white for Spdo-GFP (q) and for mCherry-dEHBP1 (r). (s-s′′′) Single confocal sections of pIIa/pIIb cells of neur^A101 strain, marked by nuclear β-gal, indicate that dEHBP1 does not colocalize with Delta punctae (arrow). (t–t′′′) Rab8YFP, expressed in pII cells by neur^Gal4, colocalizes with dEHBP1 in vesicular structures as well as at the actin-rich interphase of pII cells, as indicated by the arrows and arrowheads, respectively. Bars: (p–r) 5 µm, (all others) 10 µm.