dEHBP1 is enriched at the basolateral side of the plasma membrane and colocalizes with F-actin at the interface of pIIa and pIIb cells. (a–e′′′) Analysis of the subcellular localization of dEHBP1 along the z axis with respect to various markers of apico-basal polarity in ESO cells (marked by neur^Gal>UAS-cd8-GFP), such as Patj (a–a′′′), Arm (b–b′′′), E-cad (c–c′′′), Dlg (d–d′′′), and FasIII (e–e′′′). (f–f′′) dEHBP1 colocalizes with F-actin at the interface of pIIa and pIIb cells, marked by the nuclear β-galactosidase (β-gal) in a neur^A101 enhancer trap fly strain. (g–g′′) dEHBP1 exhibits a punctate pattern in thoracic epithelia. Arrows point to the enrichment of dEHBP1 at the interface of ESO cells (marked by neur^Gal>UAS-cd8-GFP). Bars, 10 µm. (h) Diagram depicting the relative localization of dEHBP1 with respect to markers of apico-basal polarity in ESO cells. Only the most prominent, basolateral expression of dEHBP1 with emphasis to the interface of ESO cells is shown, for the sake of clarity. (i–j′′) mCherry-dEHBP1, expressed in ESO lineages by neur^Gal4, localizes at the interphase of pII cells and within intracellular punctae, also recognized by the anti-dEHBP1 antibody. Sections are shown at the xy (i–i′′) as well as at the xz level (j–j′′).