PCMs are dependent on MTs but not on actin. (A) Projections of selected time points showing TZ nuclei expressing GFP::HIM-8 and mCherry::histone. X chromosomes are selectively labeled by incorporation of Cy5-dUTP. Animals were injected with colchicine, latrunculin A, or buffer. Bars, 1 µm. Leftmost images show the complete GFP::HIM-8 trajectory for each 5-min series overlaid on a projection of the initial z stack. Times are given in minutes and seconds. (B) Distributions of step sizes from worms injected with colchicine (left) or latrunculin A (right) compared with controls (colchicine: 2,583 steps, 46 trajectories, and 2 datasets; latrunculin A: 1,363 steps, 24 trajectories, and 1 dataset; buffer injected: 1,855 steps, 32 trajectories, and 1 dataset; bins = 0.1 µm). (C) Fraction of steps >0.6 µm observed for each condition. (D) RMSD plots for all trajectories analyzed in B and C.