GFP::HIM-8 reveals X PC dynamics. (A) Projection image of 12 optical sections from a single time point showing a field of nuclei expressing GFP::HIM-8 and mCherry::histone. Meiosis progresses from left to right. At the left edge of the field, nuclei have not yet entered meiosis. Pachytene nuclei lacking a crescent-shaped chromosome mass are seen toward the right. (B and C) Projections of selected time points showing a single nucleus expressing GFP::HIM-8 in which the X chromosome is visualized by Cy5-dUTP incorporation. Nuclear outlines are based on the mCherry::histone signal (not depicted). The dotted lines demarcate the nuclear outline, as observed by imaging of mCherry::histone. (D and E) Projections of selected time points showing GFP::HIM-8 (red) and mCherry::histone. These examples show nuclei in which X PCs (green) stretch (D) or dissociate temporarily (E) after colocalizing. Times are given in minutes and seconds. Bars: (A) 5 µm; (B–E) 1 µm.