Chromosome segregation failure in AurAKO/AurB-inhibited cells. (A) Time-lapse microscopy of H2B-GFP–expressing cells after 1NMPP1 release. Shown are still images of cells with the indicated treatments. AurB was inhibited with 60 nM AZD1152-HQPA and Mps1 with 500 nM reversine. Times are given in minutes. Bars, 10 µm. (B) Quantitative analysis of mitotic defects observed in A (n ≥ 57 cells for each condition). In addition, AurAKO and AurB-inhibited cells were transfected with Mad2 siRNA (siMad2) 24 h before the experiment. (C) Duration of mitosis (n ≥ 48 cells for each condition). (D) Cell survival analysis of cells released from 1NMPP1 and treated as indicated. Cell survival was followed for 24 h by long-term live-cell imaging and analyzed every 6 h (n ≥ 20 cells for each video, three different videos). The horizontal lines represent the mean values for each data series. Error bars indicate means ± SD. Ctrl, control; NEBD, nuclear envelope breakdown.
