Lack of Shs1 disrupts septin collar organization at the light microscope level. (A–D) Either SHS1⁺ (left) or shs1Δ (right) cells expressing either Cdc11-GFP from the CDC11 promoter on a CEN plasmid (A and C) or Cdc10-mCherry from the CDC10 promoter at the CDC10 locus on chromosome III (B and D) were examined by fluorescence deconvolution microscopy. Arrows point to aberrant septin collars. Dotted lines outline cells. Bars, 2 µm. (E) Fraction of SHS1⁺ and shs1Δ cells displaying abnormal collars with either Cdc11-GFP or Cdc10-mCherry as indicated. The data shown are from a single representative experiment out of two replicates.