Shs1 promotes formation of rings under low salt conditions. (A) A sample of Cdc11-containing complexes was diluted from high salt conditions into low salt buffer, as described in Materials and methods, applied to EM grids, and examined as in Fig. S1 B. (B) A sample of the Shs1-containing complex was treated as in A. (C–F) Time course of ring and spiral formation by Shs1-containing complexes. A sample of a preparation of Shs1-containing complexes in a high salt complex was diluted as in B into low salt at 4°C, and samples were taken at the indicated times—5 s (C), 10 min (D and F), and 60 min (F)—and immediately applied to EM grids and examined. Note the scale bar difference in F.