Fsp27 is enriched at LDCS. (A) Fsp27, but not Plin or ADRP, is enriched at the LDCS in 3T3-L1 adipocytes. The images on the right show the enlarged area of LDCSs in boxed areas on the left. (B) Untagged Fsp27 (coexpressed with HA-Plin), Fsp27-GFP, and Cidea-GFP are enriched at LDCSs when expressed in 3T3-L1 preadipocytes. Insets show enlargements of the boxed regions. (A and B) Arrowheads point to LDCSs. (C) Double immunogold labeling of Fsp27 and Plin in 3T3-L1 adipocytes by immuno-EM. (D) Images showing the kinetics of Fsp27-GFP enrichment at LDCSs. Red arrowheads point to the newly formed LDCS. (E) Fsp27 is rapidly diffused on the LD surface. Boxes represent photobleached areas. MOI in the bleached area was plotted as the percentage of the initial intensity from four independent experiments. Blue and red lines correspond to the intensity in blue or red boxed areas, respectively. Bars: (A and B) 5 µm; (C) 200 nm; (D) 4 µm; (E) 2 µm.