Eph extracellular domains mediate heterooligomerization. (A) HEK293T cells were transfected with Wt EphB2 alone (control [cont]) or with GFP-tagged EphA2 constructs, either full length (Wt) or lacking the ΔLBD or ΔCRD. EphB2 IPs were analyzed for associated GFP-EphA2 by an α-GFP WB and an α-EphB2 blot to confirm equal loading. Relative expression of GFP-EphA2 constructs was assessed by a WB of total cell lysates (bottom). (B) HEK293 cells were transfected with Wt EphB2 and AP-EphA3, full length (Wt) or lacking the ΔCRD, ΔLBD, or intracellular domain (ΔICD). EphB2 IPs were analyzed for associated AP-EphA3 by an α-AP WB. Relative expression of constructs was tested by an α-AP IP/WB and anti-EphB2 blots of total cell lysates. The graph shows relative EphA3 coIP/EphA3 expression from densitometry. A representative dataset from two independent experiments is shown.