Cartoon showing how Myo4p–She3p supports continuous motion of ASH1 mRNA. She2p binds to mRNA and escorts it out of the nucleus. In the cytoplasm, two single-headed Myo4p–She3p complexes are recruited by the She2p tetramer. The two-headed complex steps in a hand-over-hand fashion, with each head moving ∼72 nm. The processive complex transports mRNA to the bud tip. Once there, the mRNA is anchored, and the complex dissociates into nonprocessive monomers and free She2p.