Actin-pelleting assays to determine which She2p constructs are able to bind to Myo4p–She3p. SDS gels showing the supernatant (S) and pellet (P) after high-speed centrifugation of actin, Myo4p–She3p, and various She2p constructs. Myo4p–She3p cosediments with actin in the absence of MgATP; thus, She2p mutants able to bind Myo4p–She3p appear in the pellet. Molecular masses of the proteins are indicated. HC, heavy chain.