Ability of various She2p constructs to correctly localize ASH1 mRNA particles in budding yeast. (A) Representative images of correctly and incorrectly localized ASH1 mRNA. Correctly localized mRNA is found in the bud, whereas particles that are not transported remain in the mother cell. (left) Differential interference contrast [DIC]. (middle) Epifluorescence to visualize GFP-labeled ASH1 mRNA. (right) Merged images. (B) Percentage of correctly localized ASH1 mRNA particles for various She2p constructs. The indicated constructs were transformed into a she2Δ strain. Constructs lacking She2p do not localize ASH1 mRNA. WT* is the WT construct with four Cys residues (14, 68, 106, and 180) mutated to Ser. All other constructs are based on the WT* backbone. Addition of a fluorescent protein to either the N or C terminus of She2p had no effect on cellular function. Data are shown for a C-terminal fluorescent protein, but the data are identical for YFP at the N terminus. The negative control (neg) is a plasmid lacking an insert. The number of cells counted is indicated above each bar. Data were derived from at least two independent transformations per construct.