Figure 2.
Figure 2. Sti localization to the CF depends on RhoA and CR assembly. (A) Drosophila S2 cells were treated with dsRNAs directed against the bacterial kanamycin resistance gene (absent in Drosophila and used as a control) or Rho1 for 72 h and then fixed and stained to detect Sti, tubulin, and DNA. (B) S2 cells were treated with dsRNAs directed against kanamycin (control) or zipper for 84 h and then fixed and stained to detect Sti, tubulin, and DNA. Percentages on the left indicate localization of Sti. (C) S2 cells were treated with dsRNAs directed against kanamycin (control) or zipper for 84 h and then fixed and stained to detect Rho1, Sti, tubulin, and DNA (not depicted in the merged images). (D) Drosophila S2 cells were treated with latrunculin A (Lat-A) or its solvent DMSO for 1 h and then fixed and stained to detect Rho1, Sti, tubulin, and DNA (not depicted in the merged images). Bars, 10 µm.

Sti localization to the CF depends on RhoA and CR assembly. (A) Drosophila S2 cells were treated with dsRNAs directed against the bacterial kanamycin resistance gene (absent in Drosophila and used as a control) or Rho1 for 72 h and then fixed and stained to detect Sti, tubulin, and DNA. (B) S2 cells were treated with dsRNAs directed against kanamycin (control) or zipper for 84 h and then fixed and stained to detect Sti, tubulin, and DNA. Percentages on the left indicate localization of Sti. (C) S2 cells were treated with dsRNAs directed against kanamycin (control) or zipper for 84 h and then fixed and stained to detect Rho1, Sti, tubulin, and DNA (not depicted in the merged images). (D) Drosophila S2 cells were treated with latrunculin A (Lat-A) or its solvent DMSO for 1 h and then fixed and stained to detect Rho1, Sti, tubulin, and DNA (not depicted in the merged images). Bars, 10 µm.

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