Figure 1.
CR dynamics in sti mutant neuroblasts. (A and B) Kymographs of time-lapse imaging of sqh-GFP in a control stiGS9053/+ heterozygous (A) or stiGS9053/Df(3L)iro2 hemizygous mutant (B) dividing larval brain neuroblast showing the progression of cytokinesis every 30 s. Bar, 1 µm. (C and D) Equatorial cell diameter during cytokinesis measured during live imaging of control stiGS9053/+ (C; n = 15) or stiGS9053/Df(3L)iro2 mutant (D; n = 14) neuroblast divisions. Time is indicated in seconds after Sqh-GFP concentration at the cell equator. All cells imaged completed contraction within 5 min, with sti mutants showing both faster and slower contractions than the more uniform controls.