Brr6 shows no functional or physical association with nuclear pores. (A and B) Optical sections were taken through nuclei of brr6.NEGFP nup107.cherry cells. Images in A show a maximal projection of the individual 0.2-µm step size z sections shown in B. There is very little registry between the Nup107 and the Brr6 signals. (C) brr6⁺ cut11.GFP and brr6.ts8 cut11.GFP cells were grown to early log phase in EMM2 before being shifted to the restrictive temperature of 36°C for 1 h. brr6⁺ cut11.GFP cells were isolated by filtration, stained with TRITC-conjugated lectin, mixed with an equal number of brr6.ts8 cut11.GFP cells, and mounted for live cell imaging at 36°C. Maximal projections of series of optical sections in the z axis show that the intensity of the Cut11.GFP signal is not affected by the inactivation of Brr6. (D) The same as for C, except that Nup107.GFP is used in place of Cut11.GFP. Again, there is no impact of the brr6.ts8 deficiency upon the distribution of the nuclear pore component. Bars: (A and B) 2 µm; (C and D) 10 µm.