![Figure 2. High-resolution lyso/LE particle–tracking analysis in COS-7 cells. High-temporal resolution recordings (∼17 frames/s) of lyso/LE motion were processed for analysis. (A) Examples of lysosomal tracks from an uninjected cell showing normal bidirectional lyso/LE motility, a 74.1 Ab–injected cell showing long plus-end–directed travel, and LIS1 DN–injected cells showing normal minus-end–directed transport and oscillatory movement (also see Fig. S2 B). (B–G) Effect of 74.1 and DN LIS1 injection on COS-7 cell lyso/LE motility. Run length (B), percentage of motility (C), and MSD (D) are shown. (E) Net displacement/particle (= [Σ-plus runs (nm) − Σ-minus runs (nm)]/number of particles). (F) Average particle velocity is shown. All error bars represent SEM. *, P < 0.05. (G) Sample size for particle-tracking analysis.](https://cdn.rupress.org/rup/content_public/journal/jcb/195/2/10.1083_jcb.201104076/8/jcb_201104076_rgb_fig2.jpeg?Expires=1773601976&Signature=az0TBlDW0DlDP1dPNrAEnOE25d-ycFm0uk4oJV3bChXjGJqh0sCwszeGrxrQjoLOKJxks9~KZjaGD7RJqZ2bvKNRQs8rBPBnENNPR3sDZ8Y08nuliFFpnUmuynQZw11HUDO0zjgKfqqmU96Us86VxJ9lyNLGFoP5x~gyxqLeLGBqswARbT6~egcMu0R~Iq0fZJMCz49uiU2FUKjHd0epSMiBbWoKOHTl-Jamvga-2NpwSxMopUhcjecD1MziBpdBjWN9umlVTo01nUVhrXcYSfLLBTPclfJyuLhEYdx5p98nl7cZm-Qe~loU0EInmhW5Ai0ysNqyvsw~bmaaAMe3Lw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
High-resolution lyso/LE particle–tracking analysis in COS-7 cells. High-temporal resolution recordings (∼17 frames/s) of lyso/LE motion were processed for analysis. (A) Examples of lysosomal tracks from an uninjected cell showing normal bidirectional lyso/LE motility, a 74.1 Ab–injected cell showing long plus-end–directed travel, and LIS1 DN–injected cells showing normal minus-end–directed transport and oscillatory movement (also see Fig. S2 B). (B–G) Effect of 74.1 and DN LIS1 injection on COS-7 cell lyso/LE motility. Run length (B), percentage of motility (C), and MSD (D) are shown. (E) Net displacement/particle (= [Σ-plus runs (nm) − Σ-minus runs (nm)]/number of particles). (F) Average particle velocity is shown. All error bars represent SEM. *, P < 0.05. (G) Sample size for particle-tracking analysis.
