Topology of dividing and stationary cells in colonies. (A and B) Visualization of dividing, nondividing, and stationary cells. (A) Morphology of BR-F–cdc3^ts cells from liquid cultures grown for 2 h at permissive (22°C) and nonpermissive (37°C) temperature. Arrows indicate a typical morphology reached by dividing cells at 37°C. (B) Distribution of Hmg1p-GFP in dividing, nondividing, and stationary cells grown in liquid medium. (C and D) Cell topology in BR-F–Hmg1p-GFP colonies. Areas with stationary (red bars and arrows), early stationary (yellow bar and arrows), and dividing (green bars and white arrows) cells are shown. Arrows mark typical cell types. Magnified regions are marked with boxes. (C) A 1.5-d-old colony. (D) A 2-d-old colony. The detail in D4 was obtained by composing two images of neighboring fields of view.